Introduction

In the development and production of electronic products, Electromagnetic Compatibility (EMC) testing is a crucial step to ensure product quality and reliability. Electrostatic Discharge (ESD) immunity testing, as an important part of EMC testing, is used to evaluate the anti-interference ability of electronic products when subjected to electrostatic discharges. The Noisken Electrostatic Discharge Simulator ESS-B3011A series is a high-performance, multi-functional ESD testing device widely used for ESD testing of various electronic equipment. This guide will provide a detailed introduction to the usage of the ESS-B3011A series simulator, helping users better understand and operate the equipment.

1. Product Overview

1.1 Product Introduction

The Noisken Electrostatic Discharge Simulator ESS-B3011A series is a computer-controlled electrostatic discharge generator that complies with international standards such as IEC 61000-4-2 and ISO 10605. It can simulate electrostatic discharge phenomena generated when a human body or object comes into contact with or approaches electronic equipment. By connecting different electrostatic discharge guns, this device can achieve various testing modes, including contact discharge and air discharge, helping users comprehensively evaluate the ESD immunity of electronic products.

1.2 Key Features

• High Compatibility: Supports multiple electrostatic discharge guns, such as GT-30R/GT-30RA, TC-815R, TC-815S, etc., meeting different testing requirements.
• Standardized Testing: Complies with international standards like IEC 61000-4-2 and ISO 10605, ensuring the authority and comparability of test results.
• Easy Operation: The inclined front panel design facilitates operation, with well-arranged knobs and switches for convenient setting of test conditions.
• Multi-functional Testing: Provides one-key setting of IEC standard test levels, discharge detection functions, pre-check functions, etc., supporting multiple testing modes and trigger methods.
• Safe and Reliable: Equipped with multiple safety protection mechanisms to ensure the safety of equipment and personnel during the testing process.

2. Safety Precautions

2.1 User Restrictions

The ESS-B3011A series simulator is only intended for use by professional technicians who have received EMC testing training.
Untrained personnel operating this device may lead to serious consequences such as electric shocks or fires.
Individuals with electronic medical devices (such as pacemakers) are not allowed to use this device or enter the testing site.

2.2 Usage Environment

Do not use the ESS-B3011A series simulator in places where smoking is prohibited or where there is a risk of explosion.
Ensure good ventilation at the testing site and avoid running the device for extended periods in high-temperature, high-humidity, or corrosive environments.

2.3 Electrical Safety

Before use, check whether the device’s power cord is intact and properly grounded.
Do not arbitrarily replace the device’s power plug or use non-standard power cords.
When connecting or disconnecting device cables, ensure that the device is turned off and the power plug is disconnected.

2.4 Operational Safety

During operation, do not touch the discharge tip of the discharge gun.
The discharge gun must not be aimed at personnel or flammable items for discharge testing.
If any abnormalities (such as smoking or strange odors) are detected during the testing process, immediately stop the test, disconnect the power plug, and contact professionals for maintenance.

3. Detailed Explanation of Product Functions and Features

3.1 Compatible Standards and Discharge Guns

The ESS-B3011A series simulator supports multiple electrostatic discharge guns, each of which complies with specific international standards.
Users can select the appropriate discharge gun according to their testing needs.

• GT-30R/GT-30RA Discharge Guns: Comply with IEC 61000-4-2 and ISO 10605 standards and are suitable for most ESD testing scenarios.
• TC-815R/TC-815S Discharge Guns: These are existing discharge guns from Noisken that can also be used for testing with the ESS-B3011A series simulator.

3.2 Operation Panel and Switches

• Display Screen: Displays information such as current test conditions, test modes, and test results.
• Knob: Used to adjust the values of test parameters. Rotating the knob changes the parameter value, and pressing the knob switches the currently edited digit.
• Function Switches: Include the power switch, test mode selection switch, trigger method selection switch, etc. Users can select the appropriate test mode and trigger method through these switches.

3.3 Convenient Functions

• One-key Setting of IEC Standard Test Levels: By pressing the [IEC LEVEL] key, users can quickly set the test voltage levels that comply with the IEC 61000-4-2 standard.
• Discharge Detection Function: In air discharge mode, the device can detect actual discharge situations and notify the user. This function helps users confirm whether the discharge has occurred successfully.
• Pre-check Function: Performs a pre-check of the internal high-voltage power supply of the device before testing to ensure normal output voltage. This function helps reduce the failure rate during the testing process.

3.4 Radiation Level Modes

• Extra Mode (EXTRA): Reduces the radiation noise generated by the discharge gun, suitable for testing scenarios sensitive to radiation noise.
• Normal Mode (NORMAL): The radiation noise level is similar to that of previous models, suitable for general testing scenarios.
  Users can switch between radiation level modes using the [EXTRA / NORMAL] switch on the operation panel.

4. Basic Testing Process

4.1 Device Connection

• Connect the AC Power Cord: Insert the AC power cord provided with the device into the AC inlet interface on the rear of the device and the other end into a power socket with a protective grounding terminal.
• Connect the Discharge Gun: Align the high-voltage input connector of the discharge gun with the high-voltage output connector on the device and insert it. Then, rotate the safety ring on the discharge gun clockwise to secure the connector.

4.2 Test Planning

Determine the test mode (contact discharge or air discharge) and test conditions (polarity, voltage, discharge interval, and number of discharges) according to testing requirements.

• Contact Discharge Testing: Use a conical discharge tip and place the tip in contact with the test point of the Equipment Under Test (EUT) for discharge testing. It is suitable for evaluating the ESD immunity of the EUT’s housing or coupling plane.
• Air Discharge Testing: Use a circular discharge tip, move the discharge gun a certain distance away from the EUT, and then quickly approach and make contact with the EUT for discharge testing. It is suitable for evaluating the ESD immunity of the EUT’s insulating coating or insulating housing.

4.3 Basic Settings

• Set Polarity: Select the polarity (positive or negative) of the output voltage by pressing the [+／−] switch on the operation panel.
• Set Voltage, Discharge Interval, and Number of Discharges: Press the corresponding switches ([VOLTAGE], [INTERVAL], [COUNT]) and rotate the knob to set the values of these parameters. The voltage setting range is from 0.20 kV to 30.0 kV, the discharge interval setting range is from 0.05 seconds to 99.9 seconds, and the number of discharges setting range is from 1 to 999 times or continuous discharge.

4.4 Execute the Test

• Start the Test: Press the [START] switch on the operation panel to start the test. The device will output high voltage and wait for a trigger signal for discharge testing.
• Input Trigger Signal: Select the appropriate trigger method (gun trigger or controller trigger) according to the test mode. In contact discharge mode, press the trigger switch on the discharge gun for discharge; in air discharge mode, press the [TRIG] switch on the main unit for discharge.
• Observe Test Results: During the testing process, observe the test results and the status of the warning lights on the display screen. If any abnormalities (such as discharge failure or device alarms) are found, immediately stop the test and check the device status.

4.5 End the Test

• Stop the Test: Press the [STOP] switch on the operation panel to stop the test. The device will turn off the high-voltage power supply output and stop the discharge testing.
• Disconnect Device Connections: After the test is completed, first disconnect the connection between the discharge gun and the EUT, and then disconnect the AC power cord. Ensure that the device is turned off before performing these operations.

5. Advanced Functions and Settings

5.1 Automatic Identification of CR Units and Discharge Cups

The ESS-B3011A series simulator has the function of automatically identifying whether the types of CR units and discharge cups and their combinations comply with standards. When the user replaces the CR unit or discharge cup and restarts the device, the device will automatically perform identification and display the compliance standards (such as IEC 61000-4-2 Ed1.2 & Ed2.0, ISO 10605 2nd Ed., etc.). This function helps users ensure the compliance of test conditions.

5.2 Sensitivity Setting of Discharge Detection Function

In air discharge mode, the discharge detection function may fail to detect the discharge due to factors such as the impedance of the discharge channel and the charged state of the tested object. At this time, users can improve the detection success rate by adjusting the sensitivity of the discharge detection function. Press and hold the [DISCHARGE DETECT] switch for more than one second to enter the sensitivity setting mode, and then rotate the knob to select low (Lo), medium (Mid), or high (Hi) sensitivity levels.

5.3 Pre-check Function

The pre-check function is used to check whether the output voltage of the internal high-voltage power supply of the device is normal. Performing a pre-check before testing ensures that the device is in good condition and reduces the failure rate during the testing process. Place the discharge gun on an insulator and away from the device body, press the [PRE CHECK] switch to display [Chk Rdy] (check ready), and then press the [START] switch to start the pre-check. The pre-check process takes about 20 seconds. After completion, the display screen will show [Chk +OK –OK] (check successful) or error information.

6. Maintenance and Troubleshooting

6.1 Daily Maintenance

• Clean the Device: Regularly use a dry cloth to wipe off dust and dirt on the device housing and operation panel. Avoid using chemical cleaners or solvents to prevent damage to the device’s surface coating or markings.
• Check Power Cords and Connectors: Regularly check whether the power cords and connectors are intact and properly grounded. If any damage or looseness is found, replace or tighten them in a timely manner.
• Storage Environment: Store the device in a dry, well-ventilated environment without corrosive gases. Avoid exposing the device to high temperatures, high humidity, or direct sunlight for extended periods.

6.2 Troubleshooting

• ERROR 1: Discharge Gun Interlock Error
  • Cause: There is an interlock signal on the high-voltage output connector.
  • Solution: Press the [STOP] switch to clear the error and correctly connect the high-voltage connector of the discharge gun.
• ERROR 3: Trigger Error
  • Cause: The trigger switch is stuck in the input position.
  • Solution: Press the [STOP] switch to clear the error and stop the trigger switch from remaining in the input position. Try changing the trigger selection (e.g., from gun trigger to controller trigger).
• ERROR 6: High-voltage Power Supply Output Error
  • Cause: The output of the high-voltage power supply cannot be confirmed.
  • Solution: Press the [STOP] switch to clear the error and check whether the device is faulty. If the problem persists, contact professionals for maintenance.
• ERROR 8: CR Unit or Discharge Cup Identification Error
  • Cause: The CR unit or discharge cup is not connected, or the GT-30R/GT-30RA discharge gun is faulty.
  • Solution: Press the [STOP] switch to clear the error and correctly connect the CR unit and discharge cup. If the problem persists, check whether the CR unit and discharge cup are faulty and contact professionals for maintenance.

7. Specifications and Parameters

7.1 Main Parameters

• Output Polarity: Positive or negative
• Output Voltage: 0.20 kV to 30.0 kV (maximum 30.5 kV), with step settings varying according to the voltage range (0.20 kV to 10.00 kV: 0.01 kV step; 10.0 kV to 30.0 kV: 0.1 kV step)
• Repetition Period: 0.05 seconds to 99.9 seconds (±10%), manually settable (0.05 seconds to 9.99 seconds: 0.01 second step; 10.0 seconds to 99.9 seconds: 0.1 second step)
• Number of Discharges: 1 to 999 times (step of 1), or continuous discharge setting (set by further reducing the lower limit [1], displayed as [Cnt])
• Electrostatic Discharge Modes: Contact discharge mode or air discharge mode
• Radiation Level Modes: Extra mode or normal mode
• Trigger Modes: Gun trigger or controller trigger

7.2 Recommended Discharge Guns

• GT-30R/GT-30RA
• TC-815R
• TC-815S
• TC-815-330/2K
• TC-815S-330/2K

7.3 Electrical Parameters

• Charging Resistance: 10 MΩ
• Power Supply: AC100 V to AC240 V (±10%), 50 Hz/60 Hz
• Power Consumption: 75 VA
• Operating Temperature Range: +15°C to +35°C
• Operating Humidity Range: 25% RH to 75% RH (no condensation)
• Storage Temperature Range: -10°C to +50°C
• Storage Humidity Range: 0% RH to 85% RH (no condensation)
• External Dimensions: (Width) 270 mm x (Height) 263 mm x (Depth) 200 mm
• Weight: Approximately 4.6 kg

8. Warranty and Maintenance Services

8.1 Warranty Period

The Noisken Electrostatic Discharge Simulator ESS-B3011A series comes with a one-year warranty service from the date of delivery. During the warranty period, if the device fails due to non-human damage, Noise Laboratory Co., Ltd. will provide free repair or replacement services.

8.2 Maintenance Services

Noise Laboratory Co., Ltd. offers professional technical maintenance services, including fault repair, component replacement, and internal adjustments. Users can contact the nearest distributor/agent or Noise Laboratory technical support for assistance.

Introduction

The HTC PAL autosampler produced by CTC Analytics AG is a highly flexible and powerful device widely used in fields such as chemical analysis, pharmaceuticals, and environmental monitoring. As a key tool for automated sample handling and injection, the HTC PAL not only enhances analytical efficiency but also ensures the accuracy and repeatability of results. This guide provides users with a comprehensive and practical manual covering aspects from device overview, pre-operation preparations, daily operations, maintenance, to troubleshooting.

Chapter 1 Device Overview

1.1 Introduction to the HTC PAL Autosampler

The HTC PAL autosampler is a robotic system based on X, Y, and Z-axis movements, specifically designed for chromatographic analyses (such as HPLC and GC). It can automatically complete sample picking, injection, and cleaning processes. Its highly customizable configurations and flexible operating modes enable it to adapt to a variety of analytical requirements.

1.2 Device Components

The HTC PAL autosampler mainly consists of the following parts:

• X, Y, and Z-axis motion system: Responsible for the precise picking and injection of samples.
• Injection unit: Includes syringes and needles for sample aspiration and injection.
• Liquid injection valve: Controls the path of sample entry into the chromatographic system.
• Rapid cleaning station: Used for cleaning syringes to prevent cross-contamination.
• Keypad terminal: The user interface for setting up and monitoring device operation.
• Safety shield: Protects operators from potential hazards.

1.3 Technical Specifications

• Sample capacity: Depending on the configuration, it can support various trays and microplates, with sample vial capacities ranging from a few milliliters to tens of milliliters.
• Injection volume range: 10 to 100 µL (standard configuration), with a minimum of 0.5 µL and a maximum of 5000 µL (through optional configurations).
• Accuracy and repeatability: <0.5% RSD (Relative Standard Deviation) for peak areas from 10 µL to 100 µL, and <1.0% RSD for volumes <10 µL.
• Injection cycle time: Typically 20 to 60 seconds, depending on plunger speed, injection volume, and cleaning cycle.

Chapter 2 Pre-Operation Preparations

2.1 Safety Precautions

• Electrical safety: Ensure the device is properly grounded to avoid electrical shocks. Do not use damaged power cords or sockets.
• Operational safety: During operation, avoid placing hands or other objects near moving parts to prevent injuries.
• Chemical safety: Understand and comply with the MSDS (Material Safety Data Sheets) for all chemicals used, and wear appropriate protective gear.
• Device protection: Do not modify the device structure or electrical connections without authorization, as this may affect device performance and safety.

2.2 Device Unboxing and Inspection

• Confirm that all accessories are complete and undamaged.
• Check the device exterior for any visible damage.
• Verify the packing list to ensure all accessories and documentation have been received.

2.3 Device Installation

2.3.1 Installation Environment Requirements

• Temperature and humidity: The device should operate within a temperature range of 4 to 40°C and a humidity environment below 75% RH.
• Workbench: A clean, flat, and stable workbench to ensure stable device operation.
• Power supply and grounding: Provide a stable power supply and ensure proper grounding of the device.

2.3.2 Installation Steps

• Place the device: Position the HTC PAL autosampler in the predetermined location, ensuring sufficient space for operation and maintenance.
• Install the injection unit: Carefully install the injection unit according to the illustrations and steps in the manual, ensuring all connections are secure and reliable.
• Install the keypad terminal: Mount the keypad terminal near the device for easy operator monitoring and setup.
• Connect the power supply: Plug the device power cord into a compliant power socket and ensure proper grounding.
• Electrical connections: Correctly connect all electrical lines, including those for the injection valve and cleaning station, according to the illustrations in the manual.

2.4 Device Initialization

• Power on: Turn on the device power, and the keypad terminal will display the initial menu.
• System self-check: The device will automatically perform a system self-check to verify the normal operation of all components.
• Parameter setup: Set the basic parameters of the device according to actual needs, such as language, date, and time.
• Object positioning: Follow the steps in the manual to position key objects of the device (such as the tray holder and injection valve) to ensure accurate identification of component positions by the device.

Chapter 3 Daily Operations

3.1 Sample Preparation

• Sample vial selection: Choose appropriate sample vials and caps based on sample properties and analytical requirements.
• Sample loading: Accurately load samples into the sample vials, avoiding cross-contamination.
• Sample tray configuration: Configure suitable sample trays and microplates according to the quantity and type of samples.
• Barcode labeling: Affix barcode labels to sample vials for automatic identification and tracking by the device.

3.2 Method Creation and Editing

Method Creation

• Select the “Method” menu on the keypad terminal to enter the method creation interface.
• Enter the method name and select the required syringe and cycle type (e.g., LC-Inj).
• Set method parameters, including sample volume, injection speed, and cleaning steps.
• Save the method for future use.

Method Editing

• Select the method to be edited from the method list.
• Modify method parameters, such as adjusting the injection volume or cleaning time.
• Save the modified method.

3.3 Task Queue Management

Task Creation

• Select the “Task Queue” menu on the keypad terminal to enter the task creation interface.
• Select the sample tray and sample range to be analyzed.
• Choose the analytical method and set task parameters (e.g., start time, priority).
• Add the task to the task queue.

Task Initiation

• Select the task to be started in the task queue interface.
• Click the “Start” button, and the device will automatically perform the analysis according to the task settings.

Task Monitoring

• View the task status (e.g., in progress, completed, canceled) in the task queue interface.
• Pause, resume, or cancel tasks as needed.

3.4 Daily Operation Precautions

• Sample order: Ensure that the order of samples in the tray matches the settings in the task queue.
• Syringe cleaning: Regularly clean syringes to prevent cross-contamination.
• Device status monitoring: Closely monitor the device operation status to promptly identify and resolve issues.
• Data backup: Regularly back up important data and methods on the device to prevent loss.

Chapter 4 Maintenance

4.1 Daily Maintenance

• Clean the device: Regularly clean the device exterior and internal components to prevent the accumulation of dust and dirt.
• Check syringes: Regularly inspect the sealing and flexibility of syringes and replace them if necessary.
• Lubricate moving parts: Lubricate moving parts according to the recommendations in the manual to ensure smooth device operation.
• Check electrical connections: Regularly inspect all electrical connections for firmness and reliability to avoid poor contact.

4.2 Periodic Maintenance

• Replace consumables: Regularly replace consumables (such as syringe needles and seals) according to the recommendations in the manual.
• Calibrate the device: Regularly calibrate the device to ensure the accuracy and repeatability of analytical results.
• Software upgrade: Promptly upgrade the device software according to notifications from CTC Analytics AG to obtain the latest features and improvements.

4.3 Maintenance Plan

• Daily maintenance: Clean the device exterior and check the syringe status.
• Weekly maintenance: Lubricate moving parts and check electrical connections.
• Monthly maintenance: Replace consumables and calibrate the device.
• Annual maintenance: Conduct a comprehensive inspection of device performance and perform a software upgrade (if necessary).

Chapter 5 Troubleshooting

5.1 Common Faults and Solutions

5.1.1 No Detector Signal

Possible causes:

• Syringe blockage or damage.
• Incorrect installation of the injection valve needle guide or seal.
• Incorrect connection of injection valve ports.

Solutions:

• Clean or replace the syringe.
• Reinstall the injection valve needle guide and seal.
• Check and reconnect the injection valve ports.

5.1.2 Sample Not Injected

Possible causes:

• Incorrect setting of the injection needle penetration depth.
• Insufficient sample volume.
• Incorrect installation of the injection valve rotor.

Solutions:

• Adjust the injection needle penetration depth.
• Increase the sample volume.
• Reinstall the injection valve rotor, ensuring the correct orientation.

5.1.3 Injection Unit Collision

Possible causes:

• Incorrect object positioning.
• Incorrect setting of the injection needle Z-axis coordinate.

Solutions:

• Reposition objects to ensure accurate positioning.
• Adjust the injection needle Z-axis coordinate.

5.2 Advanced Troubleshooting

For more complex faults, technical support from CTC Analytics AG may be required:

• Contact technical support: When unable to resolve the fault independently, promptly contact the technical support team of CTC Analytics AG, providing a detailed fault description and device information.
• Remote assistance: Under the guidance of the technical support team, perform remote fault diagnosis and repair.
• On-site service: For faults requiring on-site repair, arrange for technicians from CTC Analytics AG to visit the site for maintenance.

Chapter 6 Advanced Features and Applications

6.1 Synchronous and Output Signals

The HTC PAL autosampler supports synchronous and output signal functions, enabling synchronous operation with other devices (such as chromatographs and data acquisition systems):

• Synchronous signals: Used to control the device to wait or continue executing sample processing steps at specific time points.
• Output signals: Used to send status or completion signals to external devices, indicating the completion of specific processing steps.

6.2 Custom Cycles and Macros

The HTC PAL autosampler supports user-defined cycles and macro functions to meet more complex analytical requirements:

• Custom cycles: Users can create custom sample processing cycles according to actual needs, including specific injection, cleaning, and movement steps.
• Macro functions: Through macro functions, users can combine multiple operation steps into a single macro command to simplify the operation process.

6.3 Multitasking and Priority Settings

The HTC PAL autosampler supports multitasking functionality, enabling the simultaneous management of multiple sample analysis tasks:

• Task priority: Users can set priorities for different tasks to ensure that important tasks are executed first.
• Task scheduling: The device automatically schedules the execution order of tasks based on task priority and start time.

Chapter 7 Conclusion and Outlook

7.1 Conclusion

This guide provides a detailed introduction to the user manual of the CTC Analytics AG HTC PAL autosampler, covering aspects from device overview, pre-operation preparations, daily operations, maintenance, to troubleshooting. Through the guidance in this guide, users can better understand and use the HTC PAL autosampler, improving analytical efficiency and result accuracy.

7.2 Outlook

With the continuous development of analytical technology, the HTC PAL autosampler will continue to play an important role in fields such as chemical analysis, pharmaceuticals, and environmental monitoring. In the future, the HTC PAL autosampler is expected to further integrate intelligent and automated functions, providing a more convenient and efficient user experience. Meanwhile, with the application of new materials and technologies, the performance and stability of the HTC PAL autosampler will also be further enhanced.

I. Introduction

The JEOL JSM-IT200 series scanning electron microscope (SEM) is a high-performance analytical instrument specifically designed for scientific research and metrology applications. This series features high resolution (below 10 nm), supports both high-vacuum and low-vacuum mode imaging, and offers X-ray energy-dispersive spectroscopy (EDS) for elemental analysis. This user guide aims to assist users in comprehensively mastering the use of the JSM-IT200 series, from safety preparations to advanced operations, ensuring efficient utilization of the instrument.

II. Safety Guidelines

Training and Protection

All users must complete Environmental, Health, and Safety (EH&S) training and wear personal protective equipment (PPE) such as safety glasses and rubber gloves.

Sample Preparation

Avoid using solvents in the SEM chamber to prevent volatile organic compound (VOC) contamination; handle plastic sharp objects properly; links to Material Safety Data Sheets (MSDS), EH&S laboratory safety manuals, and CIF safety manuals are available on the computer desktop.

Instrument Operation

Record any abnormal responses or error states, capture images using the screenshot tool, and notify relevant personnel; promptly report filament failures and replace the Wehnelt cap and spare filaments.

Vacuum System

Wear gloves when exchanging samples and keep the sample holder clean; firmly secure powder samples to prevent damage to the electron gun.

III. System Overview

The JSM-IT200 series consists of an electron optical column (EOS), a sample chamber, a vacuum system, and control software. In terms of software, log in to the instrument computer as the.\cif user and manage logins/logouts using the LockScreen program; the OperationServer icon on the taskbar is a critical background process for SEM operation; the bottom of the desktop contains main programs such as SEM Operation and SMILE VIEW Lab.

IV. Sample Preparation and Loading

Sample Preparation

Samples must be firmly fixed and have moderate conductivity; non-conductive samples require gold coating; powder samples must not be loosely loaded.

Sample Holder

Various types of holders are provided; measure the sample height to prevent collisions.

Loading Process

Follow the guidance of Specimen Exchange Navi, including venting, opening the chamber, entering sample information, setting parameters, adjusting the Z-axis height, closing the chamber door, evacuating the chamber, and starting the electron gun.

V. Software Interface and Operation

Main Interface

Displays real-time images, with the control bar below including zoom, focus, etc.; stage navigation is located in the upper right corner, and the chamber camera helps tilt samples.

Control Options

Include screen buttons and MUI knobs; the mouse wheel controls zooming; in manual mode, adjust focus and astigmatism; automatic astigmatism correction simplifies operations.

Display Histogram

Optimize brightness/contrast settings to ensure no signal clipping.

Advanced Navigation

For example, Holder Graphics displays the current sample position, and the SNS option switches to CCD color images.

VI. Imaging Techniques

Imaging Modes

Include secondary electron images (SEI) and backscattered electron images (BSE), highlighting surface topography and compositional differences, respectively.

Parameter Optimization

Adjust the accelerating voltage, probe current, and magnification according to sample requirements.

Automatic Functions

One-click adjustment of focus, contrast, brightness, and astigmatism; use BED and LSED to obtain images in low-vacuum mode.

Advanced Functions

Such as the Montage function for automated large-area observation and 3D imaging options for creating stereo images and 3D models.

VII. Elemental Analysis (EDS)

Operation

Specify points/lines/areas on the SEM screen for EDS analysis, displaying X-ray spectra and major elements in real time.

Analysis Methods

Include qualitative analysis, quantitative analysis, line analysis, and elemental mapping.

Advanced Functions

Such as QBase database comparison of spectra, PlayBack Analysis for replaying accumulated frames, and GSR analysis for automated classification of gunshot residue particles.

VIII. Data Management and Reporting

Data Management

SMILE VIEW Lab integrates the management of CCD, SEM images, and EDS data, supporting search and position display.

Report Generation

Select data to automatically layout reports, support the creation of templates and one-click updates; output to Word or PowerPoint for easy sharing.

IX. Maintenance and Troubleshooting

Maintenance

Pre-align the filament; gun alignment is fully automatic; when replacing the filament, insert the Wehnelt to automatically center it.

Troubleshooting

Record error states and take screenshots; promptly report filament failures and replace them; follow the procedure when ending a session.

X. Advanced Functions

Zeromag

Seamlessly transition from optical to SEM, supporting multi-analysis position presets and reviews.

Particle Analysis Software

Automatic/manual particle detection, EDS analysis, classification, and statistical graphs.

Language Switching

The UI supports Japanese/English; the vacuum system is fully automatic.

Installation Requirements

Specify power supply, room temperature, humidity, and space requirements.

XI. Conclusion

The JSM-IT200 series simplifies SEM operations and enhances analytical efficiency through integrated software and automatic functions. This guide covers comprehensive steps from safety to advanced applications, and users can apply them according to their specific configurations. Practice is key; by analyzing poor images, optimizing parameters, and utilizing tools such as histograms and automatic corrections, users can ensure instrument reliability and data accuracy, driving innovation in fields such as materials science and biology.

Introduction

SMILE VIEW Lab is a professional data management and analysis software specially designed by JEOL Ltd. for electron microscope systems. It supports the processing and analysis of data collected from JEOL high-end instruments such as JXA-ISP100, JXA-iHP200F, JSM-F100, and JSM-IT800. It integrates Sample Navigation System (SNS) images, Scanning Electron Microscope (SEM) images, Energy Dispersive X-ray Spectroscopy (EDS) data, and positional information, storing them in project files. This guide aims to provide comprehensive and original technical guidance to help users fully master the software from installation to advanced applications.

Software Overview and Installation

Core Functions

• Data Integration: Links sample images, electron microscope images, and EDS results, supporting graphical representation of positions.
• Analysis Tools: Offers functions such as spectrum editing, one-dimensional comparative spectra, line profiles, and pop-up spectra editing.
• Advanced Visualization: Supports 3D surface topology reconstruction, viewing at any zoom level and angle, and surface roughness standards conforming to ISO/JIS/ASME.
• Report Generation: Features an intuitive layout editor, supports PDF/Word export, and multi-page document creation.
• Compatibility: Seamlessly integrates with specific JEOL models and supports miXcroscopy™ image positioning.

Pre-installation Preparations

• System Requirements: Windows operating system (Windows 10 or higher recommended), at least 8GB RAM, Intel i5 or equivalent processor, dedicated graphics card (supporting OpenGL), and sufficient storage space.
• Software License: Non-exclusive and non-transferable; reverse engineering or copying is prohibited.

Installation Steps

1. Download the installation package (.exe file) from the JEOL official website or authorized channels.
2. Double-click the installer and select the installation path (default: C:\Program Files\JEOL\SMILE VIEW Lab).
3. Accept the license agreement and install dependent components such as .NET Framework.
4. After installation, restart the computer and activate the software with administrator privileges.
5. If integrating EDS, configure standard data and measurement conditions.

Starting the Software

Starting Methods

• Click the “Data” button through SEM Center.
• Select “Project – Data management” from the File menu.

Starting Process

1. Ensure that the JEOL instrument is connected and data has been collected.
2. Open SEM Center and navigate to the data management option.
3. Click to start, and the software loads the database, displaying the project tab panel.
4. During the first start, the software may prompt you to configure user accounts (administrator privileges are required for data sharing).

Precautions

• Avoid operating in environments with high electromagnetic interference and ensure the computer is grounded.
• Software version information can be viewed under the Help tab.

Screen Configuration

Interface Layout

• Project Tab Panel: The core data management area, including the Ribbon menu, address bar, project file list, collected data list, and sample image area.
• Favorite Tab Panel: A collection of shortcuts for quick access to frequently used projects or data.
• Report Tab Panel: The report management area, supporting preview, deletion, and export of report files.
• Layout Tab Panel: The layout editor for customizing report templates.

Ribbon Menu

• Home: Copy projects, import/export data, search, toggle display, and access the recycle bin.
• Setting: Chemical formula calculation, standard data management, EDS settings, report settings, and measurement conditions.
• Admin (Administrator Only): Data sharing and database maintenance.
• Help: Version information.

Mouse and Touch Operations

Supports click selection, right-click menu, drag-and-drop adjustment, and pinch-to-zoom. The interface supports customization, such as changing display formats or sorting data.

Data Management

Operations from the Ribbon

• Copy Project: Select a project and click Copy Project.
• Import Data: Supports importing project/sample unit jlz files.
• Export Data: Export in jlz format, supporting sample units, reports, and layouts.
• Search Data: Search for files in the project list.
• Display Toggle: Classify and display data types.
• Recycle Bin Operations: Temporarily store deleted files and support recovery.
• Data Sharing: Administrators set up sharing among users.
• Version Check: Display the software version.

Project File List Operations

Create new projects, move samples, filter and display formats, and right-click menu operations (rename, send to recycle bin, copy, export, batch analyze spectra, move to other projects).

Sample File Operations

Right-click menu operations (rename, delete, export, batch analysis, move).

Collected Data Operations

Double-click to open the analysis window, and right-click menu operations (open, delete, add to favorites, restore conditions/stage positions, add to report, save as other formats, correspondence program, particle size analysis).

Other Functions

Restore conditions, restore stage positions, add to report, save formats, correspondence program, particle size analysis.

Checking and Editing Collected Data

Opening Collected Data

Select data and double-click to display the analysis window.

Editing Sample Images

Adjust brightness, contrast, rotation, and mark positions.

Editing Electron Microscope Images

Zoom, measure distances/angles, and enhance images.

Spectrum Analysis

Edit one-dimensional comparative spectra, adjust baselines, identify peaks, and quantify.

Line Analysis

Edit profiles, smooth curves, and extract data.

Mapping Analysis

Edit pop-up spectra and adjust line profiles.

Correspondence Program (Image Alignment)

Starting Method

Select Correspondence from the data right-click menu.

Operation Steps

1. Specify Matching Mode: Automatic positioning or manual.
   • Automatic Positioning: Specify the input image, magnification (Mag), region of interest (ROI), set parameters, and run processing.
   • Manual Positioning: Manually adjust image overlay.
2. miXcroscopy™ Image Positioning: Specific integration mode.
3. Fine-tune Partial Images: Move, resize, and rotate.
4. Adjust Image Quality: Brightness and contrast.
5. Save Results: Export aligned images.
6. Scale Space Detection: Use image pyramids to optimize matching.

Report Generation

Screen Configuration

The report creation window includes a layout editor, data list, and preview.

Creating a Report

Select a template and create a new layout base.

Editing a Report

Add data, covers, and headers/footers.

Creating a New Layout

Use the layout editor to add items, adjust positions, and save.

Adding Data

Add from the list or analysis screen, supporting comparison.

Adding Covers/Headers/Footers

Customize text and page numbers.

Exporting Reports

Export as electronic data (PDF/Word) or print.

Transferring Data to Other Computers

Exporting Data

Select projects/samples/reports/layouts and generate jlz files.

Importing Data

Select jlz files and import them into new projects, supporting Windows Explorer drag-and-drop.

Precautions

Ensure compatibility and back up data before transfer.

Database Maintenance Tools

Starting/Closing

Start from the Admin tab and confirm closing.

Backup

Select the source/target and perform backup.

Restore

Restore data from backup.

Path Change

Move the data folder and use backup data.

Error Messages

Handle common issues such as invalid paths.

Troubleshooting

Common Problems

• Startup Failure: Check the license and system requirements.
• Data Import Errors: Verify the jlz format.
• Analysis Window Unresponsiveness: Restart the software and check memory.
• Report Export Failure: Confirm permissions and update the software.

Contact Information

If problems persist, contact the JEOL service office.

Software Warranty

The warranty period is 12 months, covering hardware/software failures but excluding improper operation.

Conclusion

SMILE VIEW Lab, as a key component of the JEOL ecosystem, significantly enhances the efficiency and accuracy of electron microscopy analysis. Through this guide, users can master comprehensive skills from basic operations to advanced functions. It is recommended to practice with actual data and regularly update the software to access new features. In the future, with AI integration, this software will further optimize automated analysis and drive scientific research innovation.

Chapter 1: Equipment Overview and Technical Specifications

1.1 Product Design Philosophy and Technical Positioning

The JEOL JSM-T200 series scanning electron microscope combines simplified operation, easy maintenance, and high performance, enabling even users without professional operational skills to easily obtain high-quality microscopic images. Its advantages, such as a large depth of field, a wide magnification range, and minimized sample preparation requirements, make it an effective instrument in research, quality control, and visual education fields.

1.2 Detailed Explanation of Core Technical Parameters

Electron Optical System Performance Indicators:

• Resolution: Under conditions of 25 kV accelerating voltage and a 20 mm working distance, the resolution can reach 10 nm (100 angstroms).
• Magnification: Continuously adjustable from 15x to 100,000x (15x is only available at a 48 mm working distance).
• Accelerating Voltage: Five options are provided: 2, 5, 10, 15, and 25 kV.
• Electron Gun Filament: Utilizes a pre-aligned box-type tungsten filament.
• Lens System: A three-stage condenser system (two condenser lenses and an objective lens).
• Alignment System: Mechanical alignment.
• Stigmator: Octupole electromagnetic stigmator.
• Image Shift: ±10 μm electromagnetic shift in any direction, controlled by a joystick.

Sample Stage Technical Parameters:
Centered Sample Stage (Type I):

• Sample Size Capacity: Maximum diameter of 10 mm × thickness of 10 mm.
• Movement Range: 10 mm on the X-axis and 20 mm on the Y-axis.
• Tilt Angle: Continuously adjustable from -40° to +90°.
• Rotation Angle: 360° full rotation.
• Working Distance: 20 mm.
• Sample Exchange Method: Achieved by pulling out the sample stage.

1.3 Scanning Detection System Configuration

Secondary and Backscattered Electron Detection: An integrated detector comprising a scintillator, light guide, photomultiplier tube, and collector is used.
Optional Detectors:

• Backscattered Electron Detector: Enables the acquisition of both morphological and compositional images.
• Transmission Electron Detector.
• Cathodoluminescence Detector.
• Sample Current Detector.
• X-ray Detector.

Chapter 2: Equipment Installation and Environmental Requirements

2.1 Power and Water Supply Configuration Requirements

Power Supply:

• Voltage: 100 V, 50/60 Hz, single-phase.
• Power: Basic instrument: 1.2 kVA, accessories: 0.8 kVA, totaling 2 kVA.
• Starting Current: 60 A (0.2 seconds).
• Voltage Fluctuation: No more than ±10%.
• Grounding Requirement: One terminal with a resistance of less than 100 Ω.

Cooling Water System:

• Flow Rate: 2 liters per minute (pressure range: 0.05-0.2 MPa).
• Water Temperature: 20 ± 5°C (outlet water temperature not exceeding 35°C).

2.2 Installation Environment Technical Specifications

Indoor Environmental Requirements:

• Room Temperature: 20 ± 5°C.
• Relative Humidity: Less than 80%.
  Ground Vibration:
• At 5 Hz: Less than 2 μm peak-to-peak in the X, Y, and Z directions.
• At 10 Hz: Less than 3 μm peak-to-peak in the X, Y, and Z directions.
• At 50 Hz: Less than 8 μm peak-to-peak in the X, Y, and Z directions.
  Stray Magnetic Field: Less than 0.3 μT (3 milligauss).

Chapter 3: Comprehensive Analysis of Equipment Operation Procedures

3.1 Standard Startup and Shutdown Procedures

Startup Operation Procedure:

• Turn on the faucet to supply cooling water to the microscope (water flow rate: 1.5-2 liters per minute).
• Turn on the main power switch on the distribution board and press the power switch on the left panel of the control console.
• Wait for 15-30 minutes until the magnification panel displays a reading, indicating that the column vacuum has reached a sufficient level to generate an electron beam and observe samples.

Shutdown Operation Procedure:

• Press the power-off switch.
• Turn off the main power switch.
• Wait for 10-15 minutes to allow the diffusion pump to cool to room temperature.
• Turn off the faucet.

Special Situation Handling:

• Power Failure: The microscope stops automatically. Manual reactivation is required after power restoration.
• Water Supply Failure: The microscope stops automatically. Manual reactivation is required after water supply restoration.

3.2 Technical Specifications for Sample Installation

Centered Sample Stage Installation Steps:

• Press the exhaust switch to allow air into the column.
• Wait approximately 40 seconds for the column to be fully exposed to the atmosphere.
• Insert the sample stub with the sample into the sample holder.
• Adjust the sample height adjustment screw so that the sample surface is flush with the edge of the holder.
• Secure the sample stub using the sample stub fixing screw.
• Return the sample stage to the sample chamber.
• Press the vacuum evacuation switch.

3.3 Detailed Explanation of Image Observation Techniques

Secondary Electron Image (SEI) Observation Setup:

• Set the sample stage control parameters and working distance selector.
• Set the detector panel and control panel control parameters.
• Press the accelerating voltage on button.
• Press the line scan and exposure buttons in sequence.
• Adjust the filament control knob to approximately the 11 o’clock position.
• Gradually rotate the filament control knob to approximately the 2 o’clock position.
• Observe the waveform changes on the CRT screen.
• Press the image mode button to observe the rapid exposure marker and raster.

Backscattered Electron Image (BEI) Observation:
The operation steps are the same as those for SEI observation, but press the BEI button during the initial setup and control the spot size between 12 and 3 o’clock.

3.4 Guide to Using Automatic Functions

Automatic Focusing Mode Operation Procedure:

• Use the coarse focusing control to roughly focus the image.
• Use the fine focusing control to precisely focus the image.
• Press the auto button, and the focus light on the display panel illuminates.
• When the magnification and/or field of view changes, press the auto button on the right side of the focusing panel.

Fully Automatic SEM Image Acquisition:
Images will automatically appear when the power switch is pressed under the following control settings.

Chapter 4: Technical Analysis of Photographic Recording System

4.1 Photographic Recording System Configuration

Comparative Analysis of Four Photographic Recording Systems:

• CSI-1: Standard configuration, Brownie film, 1:0.5 photographic ratio.
• CSI-2: Polaroid film pack, 1:0.75 photographic ratio (optional).
• CSI-3: 35 mm film, 1:0.25 photographic ratio (optional).
• CSI-4: Polaroid loose-leaf film, 1:1 photographic ratio (optional).

4.2 Technical Specifications for Photographic Operation

Scanned Image Photography Procedure:

• Install the recording system on the CRT and secure it with hinge pins.
• Insert the CSI connector into the socket on the display panel.
• Obtain an image on the CRT.
• Swing the CSI onto the CRT and secure it with a latch bar.

4.3 Film Selection and Parameter Settings

Relationship Between Film Sensitivity and f-value:

• 50 ASA → 5.6-8 f.
• 75, 100 ASA → 8-11 f.
• 200 ASA → 11-16 f.
• 400 ASA → 16-22 f.

Notes on the Use of Ultra-High-Speed Film:
Ultra-high-speed film (ASA 3000) is generally not suitable for image recording due to its lower resolution and narrower latitude. The use of such film should be limited to special-purpose photography, such as recording dynamic behavior.

Chapter 5: Equipment Maintenance and Troubleshooting

5.1 Key Points of Daily Maintenance

Oil Rotary Pump Maintenance:

• Regularly check the oil level and replenish as needed.

Diffusion Pump Heater Replacement:

• Turn off the power switch and the main power switch on the distribution board.
• Remove the rear panel.
• Allow the heater assembly to cool.
• Remove the heater assembly.
• Take out the heater from the cover.
• Disconnect the leads connected to the heater and remove the heater.

5.2 Technical Guidance for Component Replacement

Electron Gun Filament Replacement Steps:

• Press the exhaust switch to allow air into the column.
• Loosen the alignment screws and remove the electron gun from the column.

Chapter 6: Advanced Applications and Optimization Techniques

6.1 Advanced Imaging Techniques

Methods for Optimizing Image Contrast:

• High-Contrast Image: Rotate the contrast control knob clockwise until the exposure marker bar exceeds the standard white level bar.
• Low-Contrast Image: Rotate the contrast control knob counterclockwise.
• Automatic Brightness and Contrast Control: First obtain the optimal image brightness and contrast at approximately 1000x magnification, then press the auto control button on the control panel.

6.2 Performance Optimization Strategies

Key Points of Stigmator Correction Techniques:

• Press the image shift button.
• Set the stigmator control knob to the 12 o’clock position.
• Observe the direction of image blur and adjust the stigmator control knob accordingly.

Adjustment of Rapid Exposure Marker:
This marker has been optimized for exposure using ASA 75 film with the lens aperture set to fully open before factory shipment. Therefore, under normal circumstances, as long as the film speed and lens aperture are maintained at ASA 75 and fully open, respectively, no adjustment of the rapid exposure marker is required. However, since the optimal exposure may vary depending on the condition and nature of the sample, occasional adjustments may be necessary. In addition, adjustments will be required when taking high-contrast and low-contrast micrographs.

This user guide covers all operational procedures of the JEOL JSM-T200 series scanning electron microscope, from basic operations to advanced applications, and from routine observations to precision photography. It provides users with a complete and detailed set of operational technical guidance. By following the operational norms outlined in this guide, users can fully leverage the equipment’s performance and obtain high-quality experimental results. At the same time, regular maintenance will ensure the long-term stable operation of the equipment and extend its service life.